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Image Search Results
Journal: bioRxiv
Article Title: Oligomeric α-Synuclein induces skin degeneration in a reconstructed human epidermis model
doi: 10.1101/2021.06.17.448863
Figure Lengend Snippet: Oα-Syn triggers TNF-α and IL-1β overexpression and the release of TNF-α by the RHE. (A-D) RT-qPCR for the proinflammatory cytokines IL-6, TNF-α, IL-1β, and IL-18 2 hours after challenge, plotted as absolute values normalized by the housekeeping (GAPDH). (E) ELISA for TNF-α released in the RHE supernatant at 2 and 24 hours after the challenge. n=3 experiments, plotted as average ± SEM, analysed by paired t-test (A-D) or two-way ANOVA followed by Tukey’s post-hoc test (E); * P < 0.05, ** P < 0.01, **** P < 0.0001).
Article Snippet: Quantitative RT-PCR reactions carried out with a total reaction volume of 10 μL containing 1X of each TaqMan™ designed primers [human; TNF (Hs99999043_m1), IL-1 β (Hs01555410_m1), IL-6 (Hs00985639_m1), and
Techniques: Over Expression, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay